Drug resistant changes at the level of single cells inform combination therapy

Immunoblot analysis of cell lysates prepared from PC9 cells that were transduced with indicated shRNAs and sgRNAs. Controls: NMC – non-mammalian sequence, EGFP – EGFP sequence, Neg1 and Neg2. RPA3 is an essential gene. Vinculin served as a loading control.

对由经指定shRNAs和sgRNAs转染的PC9细胞制备的细胞裂解物进行的免疫印迹分析。对照：NMC-非哺乳动物序列，EGFP-EGFP序列，Neg1和Neg2。RPA3基因是必需基因。Vinculin作为加样对照。