Pseudouridylation of mRNA and non-coding RNAs in Arabidopsis

Pseudouridine (Ψ), the most abundant modified nucleoside, has been addressed to widely occur in mRNA and non-coding RNAs in yeast, human, mammals and the parasite Toxoplasma gondii; and its position and specificity in these RNAs are also revealed. However, the distribution and feature of Ψs in various RNAs, particularly in mRNA in plants remain elusive. In this report, we performed a genome wide and single-nucleotide-resolution pseudouridine sequencing, and identified hundreds of Ψ sites in mRNA and multiple Ψ sites in rRNAs, tRNA and other RNAs in Arabidopsis for the first time. Many predicted and novel Ψ sites in Arabidopsis rRNAs and tRNA were detected.