Analysis of kinetics of expression of the miRNA-124 precursor transcripts pri-miRNA-124.1, pri-miRNA-124.2 and pri-miRNA-124.3 in RAW264.7 cells treated with IL-41.
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Analysis_of_kinetics_of_expression_of_the_miRNA_124_precursor_transcripts_pri_miRNA_124_1_pri_miRNA_124_2_and_pri_miRNA_124_3_in_RAW264_7_cells_treated_with_IL_4_1_/878531
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in Materials and Methods. Data of four experiments performed in triplicates for each precursor is shown.1 The cells were treated with 50 ng/ml of IL-4 and harvested at indicated time points after adding of IL-4. RNA was isolated and expression of pri-miR-124.1, pri-miR-124.2 or pri-miR-124.3 was analyzed by real-time RT PCR as described “−”) if no product was detected after more than 40 cycles of amplification.2 Expression was considered negative (“+”) for CT3 Expression was considered positive (
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2015-12-02



