Single cell sequence of tumor infiltrated immune cells after cisplatin treatment in control or miR-21 KO in mouse oral cancer model

Single cell sequence (SC-seq) has been used to explore the effect of miR-21-modulated chemotherapy-induced tumor microenvironment change. MTC-Q1 WT or MTC-Q1 miR-21 KO cells were subcutaneously injected into C57BL/6 mice. CD45+ immune cells has been isolated after cisplatin injection. The isolated immune cell were sequenced by dropped based scRNA-seq, cDNA were sequenced by Illumina Novaseq 6000. Overall design: SC-seq of CD45+ immune infiltrated cell in MTC-Q1 WT or MTC-Q1 miR21 KO subcutaneous tumor after 72 hr of cisplain (5mg/kg) injection.