Parameters from global fitting of a dose-response dataset using an NMT inhibitor with HeLa cells expressing ECFP-Gag and EYFP-Gag using a bi-exponential donor FRET model.

represents the lifetimes of the donor-only decay and represents the fractional contribution of each component. represents the FRET efficiency for each component as defined in Equations 9–13. All three parameters are determined globally. represents the fluorescence lifetime of the FRET population calculated from the fitted parameters and is therefore shown in italics.