File S1 - Epigenetic Mechanisms in Respiratory Muscle Dysfunction of Patients with Chronic Obstructive Pulmonary Disease

Detailed methodologies. Figure S1 in file S1: Representative immunoblots of vinculin protein content (117 kDa) as the loading control in the diaphragms. In each fresh mini-gel, first lane from left corresponds to the molecular weight marker. All gels were always run together within the same mini-cell electrophoresis box. Definition of abbreviations: KDa, kilodaltons. Figure S2 in file S1: Representative immunohistochemical preparations corresponding to the staining of type II fibers in the diaphragms of a healthy control subject and a patient with severe COPD. (DOC)