Biochemical and photochemical mechanisms that produce different UV-induced mutation spectra

This dataset is associated with the paper entitled "<i>Biochemical and photochemical mechanisms that produce different UV-induced mutation spectra</i>", by Sugiyama, Keinard, Best, and Sanyal.<br>"Fastq.zip" contains all fastaq raw data files that have been used in the paper. Fastaq file name contains barcode # (e.g. 048.fastq) and stored in folders with NGS ID (19C, 19D etc). These identifications are used in Table S1 of the paper, which summarizes experimental conditions. <br>"NGS Protocol 4.2.docx" is our laboratory protocol for basic analysis of our NGS data. This protocol is designed for students to compute mutation frequency from fastaq data. Protocol uses four Excel template files with macro, which are also included in this dataset. The protocol and macros are designed for the specific data analysis, not for general use. Macro works only for specific template sequences used in the paper.