Deep mutational scanning of influenza H3N2 neuraminidase (A/Moscow/10/1999). The mutant library consists of all possible single amino acid mutations across residues 82 to 469.

NIAID Data Ecosystem30

Scaffold RNA engineering in type V CRISPR-Cas systems: a potent way to enhance gene expression in the yeast Saccharomyces cerevisiae.

CRISPR基因调控

酵母合成生物学

New, orthogonal transcription factors in eukaryotic cells have been realized by engineering nuclease-deficient CRISPR-associated proteins and/or their guide RNAs. In this work, we present a new kind o

Alliance of Genome Resources30

Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes

酶表达优化

酵母合成生物学

We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and man

Figshare2019-05-13 更新40

Additional file 7 of Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins

酵母合成生物学

异源表达优化

Additional file 7: Table S7. Primers used in this study.

Figshare2024-02-26 更新30