Characterisation of CFU-GM and CFU-GM-derived M-CSF-dependent macrophages by flow cytometry.
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CFU-GM cells taken immediately after their expansion in semi-solid medium were fluorescently stained by primary labelled antibodies for CD45, CD14, CD16, CD34, CD86 and CD206, and the proportion of positive cells estimated. These cells were compared with macrophages generated from CFU-GM cells by treatment with M-CSF (17 days); two independent experiments analysed in triplicate, mean ±SEM shown.
创建时间：
2013-09-12
