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Expression analysis of Prdm4 mutant and overexpressing mouse embryonic stem cells

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NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46308
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Expression profiling of wild-type, Prdm4 null and Prdm4-EGFP overexpressing mouse embryonic stem cells using Illumina whole genome V2 arrays. The hypothesis tested was that Prdm4 regulates gene expression consistant with Prdm4 genomic binding identified by ChIP-seq Prdm4 null embryonic stem cells were generated by gene targeting. Prdm4-EGFP overexpressing cells were generated through stable transfection. Total RNA was obtained from six wild-type ES cell clones, four Prdm4 null mutant ES cell clones and six Prdm4-EGFP overexpressing ES cell clones and hybridized to Illumina WG6_V2 arrays
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2019-01-16
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