The transcriptome data of immature dendritic cells were treated with hydrogen peroxide

RNA sequencing(seq-RNA) was used to analyze the underlying mechanism of immature dendritic cells (imDCs) response to extracellular reactive oxygen species (ROS). The oxidative stress model of imDCswas constructed by simulating ROS with H2O2. The final H2O2 concentration was determined by CCK8 and Annexin V-FITC/ PI double staining methods. The free migration of imDCs was analyzed by cell real-time imaging. Total RNA was extracted and transcriptome sequencing was performed. Go and KEGG enrichment analysis of differential expressed genes(DEGs) was performed. Transcripts related to metabolism and skeleton regulation were screened according to the changes of differential gene expression =1 and FDR=0.05. The result showed that there were no significant changes in cell viability and apoptosis of imDCs when H2O2 concentration was less than 100 µmol/L. The free migrate ability of imDCs was enhanced in ROS environment. RNA-seq data showed that 282 differentially expressed genes between the control group and the H2O2 treatment group. Among them, 225 differentially expressed genes were down-regulated, and 57 were up-regulated. GO and KEGG enrichment analysis found that these genes were involved in metabolism, cell cycle and cytoskeleton related signaling pathway. Gsta1, Gsta3,Cstdc5,Cstdc5 and Prdx1 involved in response to xenobtiotic metabolism were up-regulated. S100a8, Tm4sf19 and Ppbp involved in skeleton regulation were also up-regulated. While Nusap1, Stmn1, Kif20 and Prc1 were down-regulated. This study suggested that the activation of antioxidant system, cytoskeletal remodeling and cell cycle arrest may be involved in regulating the migration ability of imDCs in response to extracellular ROS. Overall design: The oxidative stress model of imDCswas constructed by simulating ROS with H2O2. The final H2O2 concentration was determined by CCK8 and Annexin V-FITC/ PI double staining methods. The free migration of imDCs was analyzed by cell real-time imaging. Total RNA was extracted and transcriptome sequencing was performed. Go and KEGG enrichment analysis of differential expressed genes(DEGs) was performed. Transcripts related to metabolism and skeleton regulation were screened according to the changes of differential gene expression =1 and FDR=0.05.