Smilax glabra flavonoids treatment of 3T3-L1 adipocytes

Smilax glabra Roxb, a traditional Chinese herb, has been widely used for folk medicine. Previous studies have found that it has various pharmacological activities, such as cytotoxic, anti-inflammation, anti-oxidant, hepatoprotective, and cardiovascular system protective activities. However, its roles in adipogenesis are poorly understood. We hypothesized that Smilax glabra Roxb and its main component may play important roles in regulating adipocyte diffrentiation and function. To test this hypothesis, we performed RNA-Seq on 3T3-L1 adipocytes treated with and without pure total flavonoids from Smilax glabra Roxb. mRNA profiles of 3T3-L1 adipocytes (8 days after differentiation) treated with and without Smilax glabra flavonoids were generated by deep sequencing, in triplicate, using Illumina HiSeq.