Pseudo first-order rate constants for CIII formation (k CIII(obs)), enzyme inactivation (ki(obs)) and heme bleaching (kb(obs)) of native VP and three mutated variants in the presence of 5000 equivalents of H2O2.
收藏Figshare2015-12-03 更新2026-04-29 收录
下载链接：
https://figshare.com/articles/dataset/_Pseudo_first_order_rate_constants_for_CIII_formation_k_CIII_obs_enzyme_inactivation_k_i_obs_and_heme_bleaching_k_b_obs_of_native_VP_and_three_mutated_variants_in_the_presence_of_5000_equivalents_of_H_2_O_2_/1398524
下载链接
链接失效反馈官方服务：
资源简介：
Reactions in 50 mM sodium tartrate, pH 5, in the presence of 5000 equivalents of H2O2. CIII formation was measured as the absorbance increase at 581 nm (Fig 8A) and heme bleaching as the absorbance decrease at 419 nm (Fig 8B). The catalytic inactivation was followed by measuring the residual activity with time, oxidizing 6 mM Mn2+ (saturating conditions) in the presence of 0.1 mM H2O2 and 0.01 μM enzyme. Means and 95% confidence limits.Pseudo first-order rate constants for CIII formation (k CIII(obs)), enzyme inactivation (ki(obs)) and heme bleaching (kb(obs)) of native VP and three mutated variants in the presence of 5000 equivalents of H2O2.
创建时间：
2015-12-03
