Genome-wide distribution of transcription-coupled nucleotide excision repair and RNA polymerase II in DRB-treated XP-C cells after UV damage

The fate of RNAPII in the course of the transcription-coupled repair pathway is unclear. To address this problem we have used methods to control transcription, so as to initiate a discrete ‘wave’ of elongation complexes. We also used methods to identify where elongation complexes and transcription-repair coupling events are located in genes throughout the genome. XP-C cells which is deficient in the global repair were treated with different DRB regimens. After UV irradiation, XR-seq and mNET were performed to analyze the repair and the localization of elongating RNA polymerase II