Transcriptome analysis of the rosettes in Arabidopsis thaliana under low nitrogen and low sulphur

The seeds of Arabidopsis thaliana Columbia Col-0 accession were sown in pots containing fine sand, and cultivated for 60 days after sowing in short days (8 h light), under control (Ctrl; 10 mM NO3- and 0.266 mM SO42-), low nitrate (Low-N; NO3- and 0.266 mM SO42-) or low sulphur (Low-S; 10 mM NO3- and 0.016 mM SO42- . For the control and Low-S conditions, each pot contained one plant; under Low-N conditions there were six plants per pot. Whole rosettes were harvested 60 days after sowing. Three independent plant replicates were obtained.