Identification of protein-degraders in an anaerobic digester by protein stable isotope probing combined with metagenomics

Presence of carbohydrates hampers the degradation of proteins in anaerobic digesters. To understand this phenomenon, we used proteogenomics to identify the active protein-degraders in the presence of low and high concentrations of carbohydrates. Active metabolic pathways of the identified protein-degraders were investigated through the application of proteomics with 13C-protein substrates (Protein stable isotope probing).