RNA-seq Analysis of control and CyPA-stimulated BEAs-2B cells Transcriptomes

Purpose: The goals of this study are to compare the difference between control and CyPA-stimulated BEAs-2B cells Transcriptomes Methods: After receiving anesthesia with pentobarbital sodium, each mouse of the WT group, hCD147 group, and hACE2 group was infected with SARS-CoV-2 by nasal drip at a dose of 3 × 105 TCID50. The lung tissues were take for RNA-seq at 2 dpi. Results: CyPA-stimulated BEAs-2B cells showed upregulated expression of a variety of proinflammatory cytokines and chemokines, including IL6, IL1b, MCP-1, CXCL1 and CXCL2, as well as several AP-1 family members, including c-FOS, FOSL1 and FOSL2 that promote the transcription of multiple cytokine genes during inflammation Conclusions: CyPA induces cytokine expression through the CD147-mediated MAPK pathway Overall design: mRNA profiles of control and CyPA-stimulated BEAs-2B cells Transcriptomes