RNA-Seq of sorted human cord-blood derived hematopoietic cell populations

The goal was to compare the transcriptome profiling of different human hematopoietic stem and progenitor populations to uncover the molecular mechanism involved in stemnes maintenance. We isolated by flow cytomtery 5 highly purified populations (long term hematopoietic stem cells (LT-HSCs), short term hematopoietic stem cells (ST-HSCs), multipotent progenitors (MPPs), common myeloid progenitor (CMPs), granulocyte and monocyte progenitors (GMPs), multilymphoid progenitors (MLPs)) from 3 to 4 cord blood pools. We extracted RNA from all these populations and performed RNA-sequencing. Raw data files will be uploaded to EGA.