Additional file 2 of Eukaryotic-driven directed evolution of Cas9 nucleases
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Additional file. 2: Table S1. Indel frequencies of selected variants. Table S2. Sanger sequences of selected variants. Table S3. Indel frequencies on genomic loci. Table S4. sgRNA cloning primers. Table S5. Cloning primers. Table S6. TIDE analysis primers. Table S7. Identified off-targets of CjCas9 WT targeting AAVS1TS1. Table S8. Identified off-targets of UltraCjCas9 targeting AAVS1TS1. Table S9. Identified off-targets of CjCas9 WT targeting AAVS1TS8. Table S10. Identified off-targets of UltraCjCas9 targeting AAVS1TS8. Table S11. Indel frequencies with mismatched sgRNAs.
提供机构:
Kheir, Eyemen; Ciciani, Matteo; Cereseto, Anna; Ruta, Giulia Vittoria; Amistadi, Simone; Casini, Antonio; Gentile, Michele Domenico
创建时间:
2024-08-15



