Raw data for Figure 3C: Mesoangioblasts and HIDEMs do not interfer with T cell activation

CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at HIDEM/mesoangioblast:PBMC = 1:4 ratio. Cells were harvested on day 3, 4, 5 or 6 and analysed for CFSE dilution and expression of CD25 and CD69. The number of CD3+7AAD- cells expressing CD25 or CD69 using counting beads and the % of CD25+ and CD69+ cells were calculated from the data. Experiments were carried out in duplicates. n=2.

CFSE 标记的 PBMCs（每孔 5 x 10^4 细胞）在 HIDEMs/中胚层祖细胞的辅助下，以 HIDEMs/中胚层祖细胞与 PBMC 的比例为 1:4 进行刺激，其中刺激物为抗 CD3/CD28 磁珠（每孔 1 x 10^4 细胞）。细胞于第 3、4、5 或 6 天收获，并分析 CFSE 稀释程度以及 CD25 和 CD69 的表达。通过计数磁珠计算表达 CD25 或 CD69 的 CD3+7AAD- 细胞数量，并从数据中得出 CD25+ 和 CD69+ 细胞的百分比。实验重复进行，重复次数为 2 次。