Real-time quantitative PCR analysis of MAPK cascade genes in brachypodium distachyon of different tissues and under multiple stresses

MAPK cascades genes responding to 14 stresses were screened using qPCR. All samples were repeated with 3 times and ACTIN was regarded as house keeping gene. The stresses included heavy metal treatments, environmental factors, phytohormones and biotic treatments. qPCR gene expression profiling. The 20d-seedlings were used and the 3µg of total RNA were sysnthesis for cDNA by using PrimeScript Reverse Transcriptase (TAKARA).