Primers used in homologous recombinations to construct knockout strain.
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The underlined sequences represent the restriction sites. Primers phoQ/phoP U-F and phoQ/phoP U-R were used to amplify upstream fragment of phoQ/phoP; phoQ/phoP D-F and phoQ/phoP D-R were used to amplify downstream fragment of phoQ/phoP.
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2015-12-02



