Determining The Ec50 Of Tracer-6908 With Acvr1/Alk2-Nanoluc
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Promega had provided us with Tracer-6908 that was developed collaboratively with SGC. Before the tracer can be used in nanoBRET assay for compound IC50, it is necessary to determine the optimal tracer concentration. The tracer concentration needs to be sufficient to produce adequate BRET signal while at the same time low enough to not obscure the competition by test compounds. The blog post for this Zenodo page is as following: https://opennotebook.thesgc.org/?p=1282&preview=true
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2018-05-28
