Comparison of microRNA expression using different preservation methods of matched psoriatic skin samples. Homo sapiens

MicroRNA expression profiling in matched lesional skin samples from 25 patients with psoriasis using the miRNA analysis platform miRCURY LNATM MicroRNA array (v. 11.0) (Exiqon). Aim: To explore the effect of three different preservation methods (Formalin-fixation paraffin-embedding (FFPE), frozen (FS) and OCT-embedding (OCT)) on miRNA expression levels in matched lesional skin samples from 25 patients with psoriasis. Overall design: Three-condition experiment, FS vs. FFPE, OCT vs. FFPE and FS vs. OCT. Biological replicates: 25 matched samples from patients with psoriasis. One replicate per array.