Table_1_Comprehensive Analysis of Circular RNAs to Decipher the Potential Roles in Blind-Side Hypermelanosis in Chinese Tongue Sole (Cynoglossus semilaevis).xlsx

Blind-side hypermelanosis is an emerging concern across the flatfish aquaculture industry including Chinese tongue sole (Cynoglossus semilaevis). Circular RNAs (circRNAs) as endogenous non-coding RNAs have been acknowledged to play important roles in various biological processes. However, the underlying regulatory mechanisms of circRNAs involved in flatfish blind-side hypermelanosis remain unclear. In this study, to profile the circRNA expression pattern and circRNA-microRNA-messenger RNA (mRNA) network, high-throughput sequencing was performed by using blind-side normal and hypermelanotic skins of tongue sole. A total of 73 differentially expressed circRNAs were identified, and the competing endogenous RNA (ceRNA) network was constructed. Furthermore, circRNA host genes and mRNAs involved in ceRNA network were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. Several GO terms and pathways of biological significance were identified and well addressed the generation of blind-side hypermelanosis to some extent. These interesting results extend the understanding of the functional profile of circRNAs and yield valuable insights into the molecular regulatory mechanisms of hypermelanosis in flatfish.

盲侧超黑素沉着症已成为包括中国舌鳎（Cynoglossus semilaevis）在内的平鱼类养殖业的关注焦点。环状RNA（circRNAs）作为一种内源性非编码RNA，已被广泛认可其在多种生物过程中的重要作用。然而，参与平鱼类盲侧超黑素沉着的circRNA的潜在调控机制尚不明确。在本研究中，为了描绘circRNA的表达模式和circRNA-微RNA-信使RNA（mRNA）网络，我们对舌鳎盲侧正常皮肤和超黑素沉着皮肤进行了高通量测序。共鉴定出73个差异表达的circRNA，并构建了竞争性内源RNA（ceRNA）网络。此外，ceRNA网络中的circRNA宿主基因和mRNA接受了基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析。鉴定出多个具有生物学意义的GO术语和通路，在一定程度上解释了盲侧超黑素沉着的发生。这些有趣的结果扩展了我们对circRNA功能特征的认知，并为平鱼类黑素沉着症的分子调控机制提供了宝贵的见解。