Gene expression profile at single cell level of leukocytes from transplanted syngeneic left lung grafts following treatment with Resolvin D1.

Neutrophils play a pivotal role in the pathogenesis of lung ischemia-reperfusion injury. The effect of pro-resolving lipid mediators, such as Resolvin D1, on early neutrophil trafficking has not been assessed in organ transplantation. We obtained single-cell RNA sequencing of leukocytes from syngeneic left lung grafts to elucidate the genetic changes induced by recipient treatment with Resolvin D1 or vehicle. Overall design: We obtained single-cell RNA sequencing of sorted CD45+ leukocytes from transplanted B6 mouse left lung grafts that were treated with intravenous Resolvin D1 or vehicle immediately prior to syngeneic transplantation. Lung grafts were exposed to severe ischemia reperfusion injury, including both cold (60 minutes at 4 degrees Celcius) and warm ischemic storage (45 minutes at 28 degrees Celcius) and were collected 2 hours after engraftment. Each condition comprised two pooled lungs.