Method of improving potexviral vector stability
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The invention provides a method of producing a potexviral vector for expressing a protein of interest in a plant, comprising producing a second heterologous nucleic acid comprising a second ORF encoding said protein and having, in the second ORF, an increased GC-content compared to a first ORF encoding said protein in a first heterologous nucleic acid, and providing said potexviral vector comprising the following segments: (i) a nucleic acid sequence segment encoding a potexviral RNA-dependent RNA polymerase, (ii) a nucleic acid sequence comprising or encoding a potexviral triple-gene block, and (iii) said second heterologous nucleic acid or a portion thereof comprising said second ORF.
提供机构:
国家林业和草原科学数据中心
创建时间:
2023-02-12



