OMAP-13: Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Pancreas with CODEX, v1.2

OMAP-13 was developed for CO-Detection by indEXing (CODEX) imaging of 4% paraformaldehyde (PFA)-fixed, cryopreserved<br>human pancreas tissue (Black et al. 2021). The panel includes 27 antibodies and DAPI for nuclear segmentation,<br>offering spatial context for 13 anatomical structures and 21 cell types described in the ASCT+B Pancreas table v1.2<br>(Campbell-Thompson, Eskaros, and Saunders 2022). It shares 8 markers with OMAP-6 (Casals and Björklund 2024) and<br>supports the identification of rare and specialized pancreatic cells (e.g., gamma and epsilon cells), lymphatic<br>endothelial cells, stromal cells, ECM components, and immune and neuronal structures. It includes markers like PPY,<br>GHRL, CD45, CD19, CD11c, IBA1, and CD14, making it suitable for imaging all regions of the human pancreas, from birth<br>to adulthood, and detecting inflammation associated with diabetes. Antibodies were validated on Akoya Biosciences'<br>PhenoCycler Open and Fusion platforms. Select antibodies (e.g., GHRL, PPY, LYVE1) were custom-conjugated; GP2 was<br>purified and conjugated by Leinco Technologies. This panel was applied to early-stage T2D samples (Walker et al.<br>2021), with image analysis performed using HALO Highplex FL (Indica Labs). Protocols and antibody metadata are<br>available on protocols.io (Saunders et al. 2022), with a representative dataset accessible on Zenodo (Saunders and<br>Reihsmann 2023) and interactive visualization provided via Pancreatlas (RRID:SCR_01856).<br><br>**Bibliography:**<br><br>* Black, Sarah, Darci Phillips, John W. Hickey, Julia Kennedy-Darling, Vishal G. Venkataraaman, Nikolay Samusik, Yury Goltsev, Christian M. Schürch, and Garry P. Nolan. 2021. “CODEX Multiplexed Tissue Imaging with DNA-Conjugated Antibodies.” *Nature Protocols* 16 (8): 3802–35. https://doi.org/10.1038/s41596-021-00556-8.<br>* Campbell-Thompson, Martha, Adel Eskaros, and Diane Saunders. 2022. “Pancreas (v1.2) Graph Data, v1.2.” 2022. https://lod.humanatlas.io/asct-b/pancreas/v1.2/.<br>* Casals, Anna, and Frida Björklund. 2024. “OMAP-6 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Pancreas with CODEX, v1.3.” https://doi.org/10.48539/HBM847.DNHP.222.<br>* Saunders, Diane, Conrad Reihsmann, Alexander Hopkirk, Marcela Brissova, and Alvin C. Powers. 2022. “CODEX® Multiplexed Imaging | Modality Overview.” May 24, 2022. https://www.protocols.io/view/codex-multiplexed-imaging-modality-overview-b6rnrd5e.<br>* Saunders, Diane, and Conrad V. Reihsmann. 2023. “Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Pancreas with CODEX.” Zenodo. https://zenodo.org/records/7916784.<br>* Walker, John T., Diane C. Saunders, Vivek Rai, Chunhua Dai, Peter Orchard, Alexander L. Hopkirk, Conrad V. Reihsmann, et al. 2021. “RFX6-Mediated Dysregulation Defines Human β Cell Dysfunction in Early Type 2 Diabetes.” bioRxiv. https://doi.org/10.1101/2021.12.16.466282.