LATS1 is a Central Signal Transmitter for Achieving Full Type-I Interferon Activity

Interferons (IFNs) possess broad-spectrum antiviral activity to resist virus epidemic. However, IFNs antiviral efficacy needs to be greatly improved. Here, we reveal that LATS1 is a vital signal transmitter governing full type-I-interferon (IFN-I) signaling activity. Importantly, LATS1 deficiency restricts IFN-I signaling and attenuates host antiviral immune response. HEK293T cells were transfected with either control shRNAs (shCON) or shRNAs against LATS1 (shLATS1) and then treated with IFNα (1,000 IU/ml) for 4 hrs. Total RNAs were isolated from HEK293T cells using TRIzol reagent (Invitrogen). The RNA samples were sent to Wuhan BGI for further sequencing and analysis.