Kinetics of osmotic stress regulate a cell fate switch of cell survival-Ramp10h_5h -ID-110320-Jurkat cells exposed to hypertonicity

To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb ramp10_5h combined comb ramp10_5h FSC_H comb ramp10_5h FSC_W comb ramp10_5h FSC_A comb ramp10_5h SSC_H comb ramp10_5h SSC_W comb ramp10_5h SSC_A combabb ramp10_5h CS_aCaspase8_PE combabb ramp10_5h BD_aCaspase3_A647 combaba ramp10_5h BD_pS6_A647 combaba ramp10_5h SC_NFAT5_A594 combabd ramp10_5h BD_pH2AX_A555 combabd ramp10_5h BD_Ki67_A700 combabe ramp10_5h BD_pNfkb_A647 combabe ramp10_5h CS_p38_PE combabg ramp10_5h BD_cPARP_A647 combabg ramp10_5h CS_aCaspase9_PE combabf ramp10_5h CS_pp38_A594 combabj ramp10_5h CS_pp38_A594 combabj ramp10_5h BD_pH3_A647 combabh ramp10_5h CS_pATF2_7_PE combabh ramp10_5h SC_SGK1_A680 combabi ramp10_5h BD_pRb_A647 combabi ramp10_5h CS_p27kip1_PE combabk ramp10_5h BD_aCaspase3_PE combabk ramp10_5h CS_CHOP_A647
Conclusion:
Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1
Notes:
timepoints 0,600,630,660,690,720,750,780,810,840,900 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=600, PO midlow + PB high=630, PO low + PB high=660, PO high + PB mid=690, PO midhigh + PB mid=720, PO midlow + PB mid=750, PO low + PB mid=780, PO high + PB low=810, PO midhigh + PB low=840, PO midlow + PB low=870, PO low + PB low=900