A Zebrafish JAK1-A634D Gain-of-Function Model Provides New Insights into the Pathogenesis of Familial Hypereosinophilia

JAK1 plays an important role during zebrafish development as hematopoietic regulator with a specific impact in the myeloid lineage, from where the eosinophils arise. Several genes in the JAK-STAT pathway are elevated in the transgenic mutants (STAT1B, SOCS3A, SOCS3B, PIAS2) as well as patways involved in hematopoiesis, immunity, response to interferon and response to biotic stimuli. Overall design: Total RNA from casper, JAK1-WT and JAK1-A634D zebrafish embryos at 28 and 36 hours post-fertilization was extracted to characterize the transcriptomic effects of the JAK1-A634D gain-of-function mutation