Gene expression analysis after selective expression of coagulation factor FVII

Analysis of coagulation factor FVII-induced breast cancer cell transformation at gene expression level. The hypothesis tested in the present study was that expression of FVII upregulates genes involved in epithelial-to-mesenchymal transition and transformation. Results provide important information on the cellular response to tumor FVII expression with respect to pro-oncogenic programs . Total RNA obtained from an MDA-MB-231-based cell line (MDA-FRT; an MDA-MB-231 cell line containing a flp-in FRT site) containing human FVII cDNA (2A3-3-flTF) compared to cells expressing an empty vector control (pcDNA).