Genome-wide profilings of chromatin accessibility and transcriptomes during different stages of G0 in fly wings

Cell cycle exit is usually enforced during terminal differentiation in various organisms and tissues. To understand how this is achieved molecularly, we exained the genome-wide profiles of chromatin accessibility and transcriptomes at normal development stages of fly wings as well as situations where we uncoupled cell cylce exit from terminal differentiation with genetic manipulations. We found that developmentally programmed, temporal changes in chromatin accessibility at a small subset of critical cell cycle genes act to enforce cell cycle exit during terminal differentiation. FAIRE-Seq and RNA-Seq on wildtype ( L3, 6h, 18h, 24h, 36h and 44h APF) and genetically manipulated (flp control, E2F1+DP, CycD+Cdk4+E2F1+DP at 24h and 44h APF) fly wings