Total RNA sequencing of mouse LSK cells in E14.5 fetal liver

The study is to obtain a transcriptome-wide map of prenatal murine stem and progenitor cells. VavCre+ mice were used for conditional knockout of Ddx41. Wild-type, Ddx41 heterozygous (Ddx41+/-) and Ddx41 knockout (Ddx41-/-) LSK cells from embryonic 14.5 day fetal liver were harvested (three samples from each group) for RNA. Gene expression analysis and alternative splicing analysis were performed. RNA-seq data were confirmed by RT-qPCR and regular PCR followed by gel quantification. A global change of gene expression and substantial differential splicing events were observed in KO samples compared to WT and het. Overall design: Total RNA profiling of E14.5 fetal liver LSK cells of WT (Ddx41+/+), het (Ddx41+/-) and KO (Ddx41-/-) mice.