Supplementary data for: Carboxy-terminal selenoproteins lack their selenocysteine active sites in the reference proteome of the farmed carp Labeo rohita

Data and code supporting a study of selenoprotein gene models in the RefSeq annotation of rohu, Labeo rohita (assembly GCF_022985175.1, annotation release GCF_022985175.1-RS_2023_02). Supplementary Data S1 (FASTA) holds the corrected protein models of the selenoprotein genes whose RefSeq records end before, or start after, the selenocysteine codon, the Selenoprofiles prediction for a selenoprotein U locus annotated only as a pseudogene, and the paired complete and truncated sequences used in the domain and motif tests. Supplementary Data S2 (ZIP) holds the record inventory, the 174-record thioredoxin reductase survey table, the Python scripts that reproduce the statistics, digestion results and figures, the SECISearch3 input and output with the predicted SECIS structures, and the output of an independent Selenoprofiles v4.5.8 run on the assembly. A README describes each file. All source sequences are public NCBI records. The published Selenoprofiles predictions used for the cross-check are not redistributed here; they are available from Ticó et al. (2026), https://doi.org/10.5281/zenodo.17828122.