Transcriptome_profiling_of_single_zebrafish_cells. Transcriptome_profiling_of_single_zebrafish_cells

Individual WT zebrafish cells were placed into lysis buffer containing a set quantity of ERCC spikes. RNA within the lysis buffer was chemically fragmented and the 3' ends of RNA was pulled down from the lysis buffer using polyT oligos attached to magnetic beads. RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5’ end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5’ end followed by 12 random bases, then an 8 base indexing tags, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 30 cycles of PCR. This data has specifically been generated to study mRNA levels in single cells. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/s