Formulation and proximate composition of the two experimental diets with (H-Chol) or without (L-Chol) cholesterol fortification.

1Experimental diet nomenclature: L-Chol diet contained no added cholesterol, H-Chol diet containing 1 g Kg−1. added cholesterol.2Basal diet composition (g Kg−1): poultry meal 211, soy protein concentrate 144, fish meal 87, blood meal 66, soybean meal 58, wheat gluten 57, whey protein 40; Ridley Agriproducts, Narangba, Queensland, Australia.3Vegetable oil: 70% linseed oil, Sceney Chemical Pty., Ltd., Sunshine, VIC, Australia and 30% Canola oil, Black and Gold, Tooronga, VIC. Australia.4Starch: Pre-gel starch, Ridley Agriproducts, Narangba, Queensland, Australia.5Min & Vit.: Complete minerals and vitamins mix supplement; Sigma-Aldrich, Inc. St. Louis, MO, USA.6Others (g Kg−1): Amino acid mix (L-Methionine, L-Lysine, glutamic acid) 3, Celite® 7, Sigma-Aldrich, Inc. St. Louis, MO, USA.7a-cellulose: alpha cellulose, Sigma-Aldrich, Inc. St. Louis, MO, USA.8Cholesterol: Sigma-Aldrich, Inc. St. Louis, MO, USA.9NFE: Nitrogen free extract calculated by difference.10Calculated on the basis of 23.6, 39.5 and 17.2 KJ g−1 of protein, fat and carbohydrate, respectively.