Comparison of Gene expression profiling of granulosa cells treated with follicle stimulating hormone or constitutively active protein kinase A

PKA activation by FSH is essential to transduce FSH-mediated effects on granulosa cell proliferation, differentiation and steroidogenesis. However, It is unknown whether activation of PKA is sufficient to account for the entire program of granulosa cell responses to FSH. We addressed this question by conducting a comprehensive comparative analysis of signaling pathways and gene expression profiles of granulosa cells stimulated with FSH or expressing a constitutively active PKA mutant, PKA-CQR.促卵泡激素（Follicle-Stimulating Hormone, FSH）介导的蛋白激酶A（Protein Kinase A, PKA）激活，对于转导FSH对颗粒细胞增殖、分化及类固醇生成的调控效应至关重要。然而，目前尚不明确PKA激活是否足以介导颗粒细胞对FSH的全部应答程序。本研究通过对经FSH刺激或表达组成型激活PKA突变体PKA-CQR的颗粒细胞进行信号通路与基因表达谱的全面比较分析，解答了这一科学问题。