Moving_C_Elegans_Corrected_Voleti_2019

Voleti, V., Patel, K.B., Li, W. et al. Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0. Nat Methods 16, 1054–1062 (2019). https://doi.org/10.1038/s41592-019-0579-4 The data is up-sampled to be isotropic with 1 pixel = 0.32 microns. It is interpolated and skew-corrected with background subtracted. Included are 3D tiff files of both red and green channels. Raw data parameters Magnification, Effective NA, configuration: ~17.73x, 0.35 NA, A_H Objective lens/Tube lens: Edmund Optics 20x/0.60NA, 200mm Frame rate (Hz), Camera: 6900 fps, Hicam Fluo Volume Rate: 25.75 vps Camera FOV (X,Y,Z)[frames/scan]: 268,1280,128 Acquired Sampling Density (X,Y,Z)[micrometers]: 1.42, 0.37, 0.32 FOV [micrometers]: 392(374), 229, 40.6 Laser Power @ sample [mW]: 9.57mW @ 488nm, 4.52mW @ 561nm