Genome-wide CRISPR/Cas9 screen of CEACAM3-mediated phagocytosis by human HL60 cells
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https://www.ncbi.nlm.nih.gov/sra/SRP433371
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资源简介:
Raw sequencing data of sgRNA sequence amplicons resulting from human HL60 cells transduced with a lentiviral genome-wide sgRNA library. Cells were selected by flow cytometry before and after phagocytosis of fluorescently-labeled CEACAM3-binding bacteria. Next, sgRNAs encoded by the selected HL60 cells were amplified by PCR and the sgRNA sequences determined via Illumina sequencing. Please see Kuiper et al. for detailed description.
创建时间:
2023-05-20



