Transcriptome Analysis of hub2-3 vs the wild-type Col. Arabidopsis thaliana

HUB2 had been previously identified as a H2B monoubiquitinating E3 ligase. Loss of HUB2 leads to a pleiotropic phenotype, including reduced seed dormancy, smaller plant size and early flowering. Microarray expression profiling was used to identify differentially expressed genes between hub2-3 mutant and wild-type Col seedlings. Overall design: The goal is to compare hub2-3 mutant to the wild-type Col. The experiment design comprised three biological replicates of each genotype.