TABLE 2 in A new species of Cyclopoliarus (Hemiptera: Auchenorrhyncha: Fulgoromorpha: Cixiidae) from American oil palms (Elaeis oleifera) in Caño Negro, Costa Rica
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TABLE 2. Primers, annealing temperatures and extension times used in this study.
LocusPrimerDirectionSequence (5’ 6 3’)AnnealingExtensionReferenceCOICOI_D1_FForwardGGAACWATAAGAAGWATAATYATYCG40˚C1 min. 30 sec.Humphries et al. 2021C1-J-2195RCReverseACTTCTGGATGACCAAAAAATCAA18S18SFForwardACTGTCGATGGTAGGTTCTG50˚C2 min.Bahder et al. 201918SRReverseGTCCGAAGACCTCACTAAA28SVForwardGTAGCCAAATGCCTCGTCA55˚C1 min. 30 sec.Cryan et al. 2000XReverseCACAATGATAGGAAGAGCC
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2025-04-05
