Tight junction integrity analysis with DextranFITC permeability assays.
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aCaco-2 cells monolayers were grown in transwell inserts and treated with wild type (WT) or Listeria adhesion protein (LAP)-expressing Lb. paracasei for 1, 4, 15, and 24 h, then treated with L. monocytogenes for 2 h. Tight junction integrity of Caco-2 cells was monitored with DextranFITC translocation across the membrane. DextranFITC recovery after L. monocytogenes was 2.68±0.03%. Values are averages of three experiments analyzed in triplicate and are significantly different between LbpWT and LbpLAP at all time points (P
创建时间：
2015-12-02
