Effects of nitrapyrin (0 to 32 micrograms per liter) on oxidation of ammonia by a diverse nitrifier-enriched microbial community

These data were collected to determine the effect of nitrapyrin, a commercial nitrification inhibitor, on nitrification by a diverse nitrifier-enriched microbial (NEM) community. The NEM community was originally collected from an aquaculture pond in Columbia, Missouri and cultured in HEPES-buffered, minimal mineral salts media, supplemented with a nominal dose of 2 milligrams per liter ammonia-nitrogen, added as ammonium-sulfate. NEM were exposed to nitrapyrin (0 to 32 micrograms per liter) for 192 hours. Before dosing, the nitrapyrin was dissolved in dimethyl sulfoxide (DMSO). After dosing, the DMSO concentration in the flasks was 0.1 percent volume to volume. Ammonia, nitrite, nitrate, pH, dissolved oxygen, temperature, water hardness, conductivity, alkalinity, turbidity, and nitrapyrin were monitored at intervals during the experiment.