Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera

In this study, we detailed the indicators of development of H. armigera, such as growth and development period, number of surviving larvae, body weight, pupa weight, and number, as well as the relative expression levels of HaSumf1 gene at different instars, and the third- and fifth-instars after feeding with glucosinolates and /or dsSumf1.

Methods
(1) H. armigera Rearing

(2) Bioinformatics Analysis

(3) Total RNA Extraction, cDNA Synthesis, and HaSumf1 Determination in Different Stages

(4) Cloning and Sequencing HaSumf1

(5)  Synthesis of dsRNA in Vitro

(6) Effects of Silencing HaSumf1 on the Growth and Development of H. armigera

(7) Gene Expression after Silencing HaSumf1

(8) Statistical Analysis