Genome-wide profile of RBPJ, H3K4me1, H3K4me3 and H3 in mouse brain pericytes

Background: Pericytes are capillary-associated mural cells especially prominent in the central nervous system where they regulate vascular permeability and blood-brain barrier integrity. Despite their relevance for neurovascular unit homeostasis and their involvement in the pathobiology of neurodegenerative diseases, signalling mechanisms responsible for functional specialization and intercellular communication remain elusive. Objectives: The main goal of this study is to understand the relevance of Rbpj (the common downstream mediator of Notch signalling, an important mediator of cell-to-cell communication) in brain pericytes. In particular, the ChIP-Seq results aimed at the identification of genes bound to RBPJ in cultured mouse brain pericytes. Overall design: Mouse pericytes (RD1) were analyzed by ChIP-Seq using an RBPJ-specific antibody to identify the genomic sites bound RBPJ. Subsequently, ChIP-Seq versus H3K4me1 and H3K4me3 were used to discriminate between proximal and distal RBPJ binding sites, respectively. Two replicates of each sample are included.