N6-isopentenyladenosine (i6A) Modified nucleotide in tRNA in budding yeast (PRJCA016586)

NIAID Data Ecosystem2026-05-10 收录

RNA修饰检测

酿酒酵母RNA分析

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https://www.ncbi.nlm.nih.gov/sra/DRP014178 数据链接链接失效反馈

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In this project, we developed a new method to identify N6-isopentenyladenosine (i6A) Modified nucleotides in budding yeast tRNA with single-base resolution.

创建时间：

2025-11-18

Computational methods for RNA modification detection from nanopore direct RNA sequencing data

RNA修饰检测

纳米孔测序数据

The covalent modification of RNA molecules is a pervasive feature of all classes of RNAs and has fundamental roles in the regulation of several cellular processes. Mapping the location of RNA modifica

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Comprehensive identification of diverse ribosomal RNA modifications by targeted nanopore direct RNA sequencing and JACUSA2

RNA修饰检测

纳米孔测序

Ribosomal RNAs are decorated by numerous post-transcriptional modifications whose exact roles in ribosome biogenesis, function, and human pathophysiology remain largely unknown. Here, we report a targ

DataCite Commons2025-02-14 更新30

Detection of SINEUP RNA modification by Oxford Nanopore direct-RNA sequencing

RNA修饰检测

Nanopore测序

To detect the modifed bases in SINEUP RNA, we compared chemically modified in vitro transcribed (IVT) SINEUP-GFP RNA and in-cell transcribed (ICT) SINEUP RNA from SINEUP-GFP and sense EGFP co-transfec

NIAID Data Ecosystem20

Pseudouridine profiling reveals widespread regulated mRNA pseudouridylation in yeast and human cells. Pseudouridine profiling reveals widespread regulated mRNA pseudouridylation in yeast and human cells

RNA修饰检测

表观转录组学

We report the development of Pseudo-Seq, a sequencing based technique for identification of pseuoduridine (Ψ) modifications within RNA. We performed Pseudo-Seq on S. cerevisiae and HeLa cells and find

NIAID Data Ecosystem40

Dts-seq: a simple method of library preparation for a highly reproducible characterization of the tRNA epitranscriptome by deep sequencing

RNA修饰检测

表观转录组学

High-throughput sequencing of cellular tRNAs is severely hindered by the presence of base modifications. These modifications impair the reverse transcription (RT) enzyme and prevent a large fraction o

NIAID Data Ecosystem20

N6-isopentenyladenosine (i6A) Modified nucleotide in tRNA in budding yeast (PRJCA016586)

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