Fugal endophyte-derived root microbiota reshaping contributes to resistance enhancement and growth promotion of peanuts

Plant probiotics usually have long-term impacts on the host. Our previous studies have shown that the fungal endophyte Phomopsis liquidambaris promotes multiple plant properties that benefit plant fitness. However, the mechanism of the fungal endophyte-derived beneficial traits remains unclear. Since the close connections between plant endophytic microbiome and plant health and resistance, thus, this study aims to clarify the role of endophytic bacterial communities in probiotic-plant interactions. Here, through amplicon sequencing and bacterial cultivation, we found that Ph. liquidambaris inoculation altered the root endophytic bacterial composition and improved the core microbial-based interaction network. Next, we assembled and simplified a synthetic community with plant growth promotion and disease resistance function based on Ph. liquidambaris-derived root bacterial community including Bacillus sp. HB1, Bacillus sp. HB9, Burkholderia sp. MB7, Pseudomonas sp. MB2, Streptomyces sp. MB6, and Bradyrhizobium sp. MB15. Furthermore, the simplified synthetic community was determined to suppress root rot caused by Fusarium oxysporum, promote peanut growth, and significantly increase the yield of peanuts suffering continuous monocropping. Collectively, our results suggest that the adaptability in the adversity of plants was attributed to Ph. liquidambaris-derived legacy effects on the peanut root bacterial community.