PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [CUT&Tag, CUT&RUN]

To understand the functional role of PRC1 in defining the epigenomic and transcriptional landscape in Aundiff, we eliminated PRC1 function in germ cells using Ddx4-Cre to mutate the E3 ubiquitin ligase RNF2 on a background lacking its partially redundant paralog RING1 (RING1A) to create PRC1 conditional knockout (PRC1cKO) mice. We discover a key role of Polycomb complexes in shielding the undifferentiated state of mouse adult spermatogonia. CUT&RUN or CUT&Tag for H2AK119ub, H3K27me3 and H3K4me3 in WT, PRC1ctrl and PRC1cKO spermatogonial cells. Overall design: Comparative chromatin profiling analysis of quantitative CUT&Tag or CUT&RUN data for (1) WT Aundiff and Adiff spermatogonia; (2) PRC1ctrl and PRC1cKO Aundiff spermatogonia; (3) PRC1ctrl and PRC1cKO Aundiff spermatogonia after win18,446 inhibition.