High-throughput exploration of single-cell transcriptional alterations associated with remote fear memory formation
收藏Alliance of Genome Resources2026-08-01 收录
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Leveraging SPEED in tandem with snRNA-Seq, we uncover molecular identities of distinct neural subtypes, concurrent 5hmC alterations at active enhancers and gene bodies, and dynamic gene expression associated with remote memory formation. This comprehensive analysis encompasses several thousand cells from the cerebral and cerebellar cortex of home-caged (HC) and fear-conditioned (FC) mice. The isolated and purified nuclei were subjected to single-nuclei sequencing utilizing the Chromium Next GEM Single Cell 5 Reagent Kits v2 from 10 Genomics.
